Exonuclease Domain-Containing 1 Enhances MIWI2 piRNA Biogenesis via Its Interaction with TDRD12
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چکیده
منابع مشابه
PIWI Slicing and EXD1 Drive Biogenesis of Nuclear piRNAs from Cytosolic Targets of the Mouse piRNA Pathway.
PIWI-interacting RNAs (piRNAs) guide PIWI proteins to suppress transposons in the cytoplasm and nucleus of animal germ cells, but how silencing in the two compartments is coordinated is not known. Here we demonstrate that endonucleolytic slicing of a transcript by the cytosolic mouse PIWI protein MILI acts as a trigger to initiate its further 5'→3' processing into non-overlapping fragments. The...
متن کاملMIWI2 and MILI Have Differential Effects on piRNA Biogenesis and DNA Methylation
In developing male germ cells, prospermatogonia, two Piwi proteins, MILI and MIWI2, use Piwi-interacting RNA (piRNA) guides to repress transposable element (TE) expression and ensure genome stability and proper gametogenesis. In addition to their roles in post-transcriptional TE repression, both proteins are required for DNA methylation of TE sequences. Here, we analyzed the effect of Miwi2 def...
متن کاملSomatic piRNA biogenesis.
Piwi-interacting RNAs (piRNAs) protect animal germ cells from transposons and other selfish genetic elements. Of the three types of animal small-silencing RNAs—smallinterfering RNAs (siRNAs), microRNAs (miRNAs), and piRNAs—piRNAs are the least well understood, because we lack good tools for studying how they are made and how they function. Brennecke et al have now established a method for trigg...
متن کاملTdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline.
Piwi proteins and Piwi-interacting RNAs (piRNAs) repress transposition, regulate translation, and guide epigenetic programming in the germline. Here, we show that an evolutionarily conserved Tudor and KH domain-containing protein, Tdrkh (a.k.a. Tdrd2), is required for spermatogenesis and involved in piRNA biogenesis. Tdrkh partners with Miwi and Miwi2 via symmetrically dimethylated arginine res...
متن کاملA systematic analysis of Drosophila TUDOR domain-containing proteins identifies Vreteno and the Tdrd12 family as essential primary piRNA pathway factors.
PIWI proteins and their bound PIWI-interacting RNAs (piRNAs) form the core of a gonad-specific small RNA silencing pathway that protects the animal genome against the deleterious activity of transposable elements. Recent studies linked the piRNA pathway to TUDOR biology as TUDOR domains of various proteins bind symmetrically methylated Arginine residues in PIWI proteins. We systematically analy...
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ژورنال
عنوان ژورنال: Cell Reports
سال: 2018
ISSN: 2211-1247
DOI: 10.1016/j.celrep.2018.08.087